Optogenetics and calcium imaging have advanced the field of neuroscience by making it possible to manipulate and image single-cell neural activity in vivo. The ability to perform cellular-resolution optogenetics and imaging in multiple brain regions (surface and/or deep-brain) enables researchers to stimulate and visualize neurons, individually or simultaneously, to study the link between neural activity in multiple brain regions. Using the methodology depicted below, researchers are able to investigate neural activity in multiple regions in the cortex and/or in the deep-brain. Below you can find application examples.