Mightex’s market-leading Polygon DMD Pattern Illuminator is designed and developed to meet these challenging requirements: it enables researchers to precisely deliver light to target individual neurons, multiple ROIs or large-scale areas, for optogenetic stimulation of one or more opsins in cultured cells, brain slices, explants, organoids, or other tissues or models.
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In unanesthetized mouse pups, layer I interneurons expressing 5HT3a serotonin receptor and reelin exhibit synchronized spontaneous calcium transients, activated by whisker stimulation. Thalamic input attenuation through NMDA receptor knockdown in these interneurons results in abnormal barrel map formation and deficits in whisker-dependent behavior, underscoring the critical role of specific interneuron types in somatosensory circuit assembly. For this study, optogenetic stimulation of patched cells was done using Mightex’s Polygon DMD pattern illuminator.
Alicia Che & Prof. Natalia V. De Marco Garcia
Cornell University, Ithaca, NY, USA
Spinal V1 neurons inhibit motor targets locally and sensory targets distally (Current Biology 2021).
In larval zebrafish, V1 (Eng1+) neurons, a major inhibitory population with dual sensory and motor functions, exhibit long ipsilateral axons spanning approximately 6 spinal segments. Using a combination of single-cell labeling, optogenetics, and electrophysiology, the researchers mapped connectivity from V1 neurons to motor and sensory spinal populations. Mightex’s Polygon DMD device was used to sequentially project 16 squares in a 4×4 grid for optogenetic stimulation.
Mohini Sengupta & Prof. Martha W. Bagnall
WUSTL, St. Louis, MO, USA
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