Overview
These are omic profiling methods that use photo-uncaging or photo-crosslinking of oligodeoxynucleotides and enable isolation of omic information from locally defined areas upon spatially-restricted photo-irradiation.
Key System Requirements
- Quick and easy integration with existing microscope setups
- Targeted stimulation of arbitrary shapes to specifically irradiate tissue, cellular and/or subcellular features
- Compatibility with UV illumination (~365nm)
- Easy software control to adjust light intensity and patterns shape and size
Polygon DMD Pattern Illuminator
Mightex Polygon DMD patterned illuminator has proven to be an ideal tool for Light-directed Spatiotemporalomics, Photo Isolation Chemistry and Light-Seq (see publications below). Please contact our applications specialists to discuss your needs and find out more about our options.
PIC/Light-Seq Research Highlights using Polygon DMD Pattern Illuminator
High-depth spatial transcriptome analysis by photo-isolation chemistry (Nature Communications 2021).
Dr. Mizuki Honda & Prof. Yasuyuki Ohkawa
Kyoto University, Kyoto, Japan
Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing (Nature Methods 2022).
Dr. Jocelyn Y. Kishi & Prof. Peng Yin
Harvard Medical School, Boston, MA, USA
Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections (Cell Press 2022).
Dr. Mizuki Honda & Prof. Shinya Oki
Kyoto University, Kyoto, Japan
